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 >  其他蛋白>Spike protein >SPN-P52H3

Pangolin coronavirus hCoV-19/pangolin/Guangdong/1/2019 Spike Trimer (R677A, KV978-979AA), His Tag

分子别名(Synonym)

Spike,S protein,Spike glycoprotein,S glycoprotein

表达区间及表达系统(Source)

Pangolin coronavirus hCoV-19/pangolin/Guangdong/1/2019 Spike Trimer, His Tag (SPN-P52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 10 - Pro 1205 (Accession # A0A7D6TQ96-1(R677A, KV978-979AA)).

Predicted N-terminus: Ala 10

Request for sequence

蛋白结构(Molecular Characterization)

Online(Ala 10 - Pro 1205) A0A7D6TQ96-1 (R677A, KV978-979AA)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 137.6 kDa. The protein migrates as 170-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Pangolin coronavirus hCoV-19/pangolin/Guangdong/1/2019 Spike Trimer, His Tag (Cat. No. SPN-P52H3) SDS-PAGE gel

Pangolin coronavirus hCoV-19/pangolin/Guangdong/1/2019 Spike Trimer, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Pangolin coronavirus hCoV-19/pangolin/Guangdong/1/2019 Spike Trimer, His Tag (Cat. No. SPN-P52H3) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-8 ng/mL (Routinely tested).

Protocol

背景(Background)

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

 

前沿进展

 
 
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Spike protein靶点信息
英文全称:Coronavirus spike glycoprotein
中文全称:冠状病毒刺突糖蛋白
种类:Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
上市药物数量:11详情
临床药物数量:39详情
最高研发阶段:批准上市
前沿进展

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