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SA-Biotin系统

1963年，Stapley首次从阿维丁链霉菌(Streptomyces avidinii)培养液中分离到的链霉亲和素 (Streptavidin，SA)。作为已知自然界中最强的非共价相互作用之一，SA同源四聚体与生物素（Biotin）可以以高亲合力牢固结合并产生多级放大效应，这使得SA- Biotin系统成为生物化学实验中被广泛应用的宝贵资产。

SA-Biotin系统常被偶联到抗体或蛋白，其中SA偶联标记物如HRP、荧光基团等，Biotin被偶联到抗体或蛋白（MABSOL®生物素标记蛋白），通过SA-Biotin桥连来实现间接检测靶标，广泛用于微量抗原、抗体定性、定量检测及定位观察研究的新技术，使得免疫标记和有关示踪分析更加灵敏。

ACROBiosystems可提供SA系列dexiconelektrik.com：重组SA蛋白，标记SA蛋白，SA包被板，SA磁珠等，助力您的科研项目和药物研发。

SA系列dexiconelektrik.com

分子	dexiconelektrik.com分类	货号	dexiconelektrik.com描述	订购/预购
Streptavidin	SA蛋白	STN-N5116	Recombinant Streptavidin	订购
	标记SA蛋白	STN-NH913	Streptavidin-HRP	预购
		STN-NA114	Streptavidin-Acridine ester	预购
		STN-NA118	Streptavidin-Alexa Fluor 488	预购
		STN-NA115	Streptavidin-Alexa Fluor 555	预购
	SA包被板	SP-11	Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame	订购
		SP-13	Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame (For Serological Testing)	订购
		SP-14	Streptavidin Coated Plates, Clear, 96-Well, Clear Frame	预购
		SP-15	Streptavidin Coated Plates, Clear, 96-Well, Clear Frame (For Serological Testing)	预购
	SA磁珠	SMB-B01	Magnetic Beads™ Streptavidin	订购

SA系列dexiconelektrik.com验证数据

SA蛋白活性（ELISA）

Immobilized Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a with a linear range of 0.1-6 ng/mL (QC tested).

申请 Protocol

Immobilized Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (Routinely tested).

申请 Protocol

SA 包被板应用数据

Immobilized Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Anti-FMC63 antibody with a linear range of 0.1-3 ng/mL (QC tested).

申请 Protocol

Immobilized Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F4) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Human PD-L1, mFc Tag with a linear range of 0.4-6.25 ng/mL (QC tested).

申请 Protocol

Immobilized Biotinylated Human CD20 Full Length, His,Avitag (HEK293) (Cat. No. CD0-H82E5) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Rituximab with a linear range of 78-625 ng/mL (QC tested).

申请 Protocol

Immobilized Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-H82W7) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Ipilimumab with a linear range of 78-1250 ng/mL (QC tested).

申请 Protocol

SA 磁珠应用数据

Immobilized 40 μg SARS-CoV-2 S1 protein to 1mg Beads （Cat. No. SMB-B01） can bind the Anti-SARS-CoV-S protein RBD Antibody with an EC50 1.157 μg/mL (QC tested).

申请 Protocol

生物素标记蛋白

参考文献

Louis Chaiet, Frank J. Wolf. The properties of streptavidin, a biotin-binding protein produced by Streptomycetes, Archives of Biochemistry and Biophysics (1964). https://doi.org/10.1016/0003-9861(64)90150-X.

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Streptavidin（SA）系列dexiconelektrik.com

SA蛋白活性（ELISA）

SA 包被板应用数据

SA 磁珠应用数据

生物素标记蛋白

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